Phytochemical Evaluation & Pharmacological Screening of Antidiabetic Activity Using Ethanolic Extracts of Pulicaria Dysenterica & Brosimum Alicastrum on Experimental Animals (2025)

Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts

Molecules, 2020

Pueraria lobata (Wild.) Ohwi. (P. lobata) flowers known as ‘Kudzu flower’ contain isoflavonoids and essential oil components. They have a wide range of biological and pharmacological activities, including protective effects against non-alcoholic fatty liver disease, hyperglycemia, and hypolipidemia, anti-mutagenic effects, and benefits for weight loss. However, the molecular mechanism of these effects remains unclear. Our study aimed to systematically examine the effects of flos puerariae crude extract (FPE) as an anti-diabetic agent using in vitro assays. The cytotoxicity of FPE was evaluated using MTS assay in L6 rat myocyte and 3T3-L1 murine fibroblast cell lines. PPARγ binding activity and adipogenesis were examined using dual-luciferase and differentiation assays, respectively. For investigating the anti-diabetic activity, glucose utilization, including GLUT4 protein expression, glucose uptake assay, and GLUT4 translocation using immunofluorescence microscopy were conducted in ...

Hypoglycemic, analgesic and anti-inflammatory effects of Pulicaria incisa (Lam.) DC extracts in animal models

Tropical Journal of Pharmaceutical Research

Purpose: To investigate acute toxicity, hypoglycaemic, analgesic and anti-inflammatory activities of Pulicaria incisa (Lam.) DC.Methods: Acute toxicity analysis was assessed by adopting the OECD - 423 Guidelines. The hypoglycemic effect of P. incisa infusion was investigated utilizing Oral Glucose Tolerance Test (OGTT) on orally glucose-induced hyperglycemic rats. The analgesic effect was investigated using the writhing test model, and anti-inflammatory activity was ascertained by carrageenan-induced paw oedema method.Results: The results showed that half-maximal lethal dose (LD50) values in mice were higher than 14 g/kg. The results of OGTT showed that the plant infusion produced a significant (p < 0.05) reduction in the glucose level in the blood post oral glucose administration. Treatments with diethyl ether, n-butanol and free aglycone extracts at 400 mg/kg significantly reduced (p < 0.05) the abdominal cramps by 82.45, 74.36 and 65.35, % respectively. Diethyl ether extrac...

Phytochemical Analyses and in vitro Anti-Diabetic Activity of Ten Indigenous Plants

Deleted Journal, 2024

DPP-IV Inhibitory Potential of Methanolic Extract of Pueraria Tuberosa in Liver of Alloxan Induced Diabetic Model

Biosciences Biotechnology Research Asia, 2018

DPP-IV is usually found to be over expressed in many pathological conditions of Liver via disturbing immune system, lipid accumulation, ECM degradation etc. The main objective of this work was to explore the effect of methanolic extract of Pueraria tuberosa (PTME) against Alloxan induced liver damage with respect to its potential of inhibiting DPP-IV activity. PTME was prepared through continuous soxhlet extractor. Alloxan injections were given to the same age group with weight range of 80-100 g Charles foster albino male rats at the dosage of 120mg/kg bw. Rats were divided into five groups. Group 1 was given with PTME dose of 20mg/100 g bw for 7 days, Group 2 for 14 days, Group 3 for 30 days, Group 4 for 40 days and Group 5 taken as an Alloxan control. Animals were sacrificed at their respective time along with the normal rats. DPP-IV activity in liver homogenates were done through the kit based on fluorescence ELISA. After treatment with PTME (20 mg/100 g bw) at different time int...

The Tuber Extract of Pueraria Tuberosa Linn. Competitively Inhibits DPP-IV Activity in Normoglycemic Rats

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective : The main objective of this research was to explore the effect of the polar fraction of tubers of Pueraria tuberosa (PTWE) on DPP-IV activity. Methods : The comparison of in vitro inhibitory potential between commercially available Galvus (Vildagliptin) and PTWE was determined by measuring percent inhibition and IC 50. The enzyme kinetics were also done to reveal the nature of inhibition. In vivo study was done via the glucose tolerance test and by the measurement of increased plasma GLP-1 concentration and DPP-IV activity after glucose load. Results: PTWE has given the IC 50 value of 17.4 mg/ml and was found to be a competitive inhibitor having the Ki value of 13.11 mg/ml. Plasma GLP-1 concentration was increased and DPP-IV activity was decreased after 60 minutes of glucose load in PTWE treated rats as compared to control rats. Overall PTWE was found to be less potential DPP-IV inhibitor than Galvus. Conclusion : These findings suggest that antidiabetic effect of PTWE co...

In Vitro and in Vivo Evaluation of Antidiabetic Activity of Leaf Essential Oil of Pulicaria inuloides-Asteraceae In Vitro and in Vivo Evaluation of Antidiabetic Activity of Leaf Essential Oil of Pulicaria inuloides-Asteraceae

The essential oil of leaf of P. inuloides was extracted by using hydrodistillation process and the volatile components investigated with the help of gas chromatography coupled with mass spectrometry. Diabetic rats were treated with a 400 mg/kg dose of essential oil of P. inuloides orally for 3 h (acute) or 21 days (chronic). In acute model blood glucose levels were monitored at specific intervals. In the chronic model blood samples were collected from overnight fasted diabetic rats on day 21 to estimate blood glucose level. And the body weight, serum lipid profile and activities of liver and kidney enzymes were measured. Histopathological observations of liver sections were also studied. Total seventy compounds were identified as 2-Cyclohexen-1-one, 2-methyl-5-(1-methylethyl)-, (S)-58.19%; Oxirane, 2-ethyl-2-methyl-8.25%; 1,2-Benzenedicarboxylic acid, diisooctyl ester 13.18%; n-Hexadecanoic acid 2.12% by gas chromatography coupled with mass spectrometry. Treatment of diabetic rats with 400 mg/kg of P. inuloides proved to have significant (P < 0.05) antihyperglycemic effect and have the capacity to correct the metabolic disturbances associated with diabetes. Histopathological studies showed that the essential oil of P. inuloides reinforced the healing of liver.

Bioassay-Guided Antidiabetic Study of Phaleria macrocarpa Fruit Extract

Molecules, 2012

THE TUBER EXTRACT OF PUERARIA TUBEROSA LINN. COMPETITIVELY INHIBITS DPP-IV ACTIVITY IN NORMOGLYCEMIC RATS Original Article

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: The main objective of this research was to explore the effect of the polar fraction of tubers of Pueraria tuberosa (PTWE) on DPP-IV activity. Methods: The comparison of in vitro inhibitory potential between commercially available Galvus (Vildagliptin) and PTWE was determined by measuring percent inhibition and IC50. Results: PTWE has given the IC The enzyme kinetics were also done to reveal the nature of inhibition. In vivo study was done via the glucose tolerance test and by the measurement of increased plasma GLP-1 concentration and DPP-IV activity after glucose load. 50 Conclusion: These findings suggest that antidiabetic effect of PTWE could be because of its role in DPP-IV inhibition. value of 17.4 mg/ml and was found to be a competitive inhibitor having the Ki value of 13.11 mg/ml. Plasma GLP-1 concentration was increased and DPP-IV activity was decreased after 60 min of glucose load in PTWE treated rats as compared to control rats. Overall PTWE was found to be less potential DPP-IV inhibitor than Galvus.

Antioxidant, Antidiabetic and Anti-inflammatory Potential of Phoenix pusilla Root- An Unexplored Folklore Plant.

IOSR Journals , 2019

Medicinal importance of Phoenix pusilla is unexplored. In the present study, antioxidant potential, α-amylase and α-glucosidase inhibitory effect of ethanol root extract were evaluated. Also the antiinflammatory potential was checked by two invitro methods. IC50 values of the extract against enzymes showed the ability of lowering glucose level (post prandial) at low concentration. The inhibitory action was high against α -amylase than towards α-glucosidase. Similarly the anti-inflammatory assays showed 88.62% and 67.51 % of inhibition. Thus, in vitro study revealed that the root extract have potential to act against free radicals and posess hypoglycemic and anti-inflammatory activity as determined by membrane stabilisation and proteinase inhibitory activity.

Active phytochemicals of Pueraria tuberosa for DPP-IV inhibition: in silico and experimental approach

Journal of Diabetes & Metabolic Disorders, 2017

BACKGROUND: We had earlier reported that the extract of Pueraria tuberosa significantly inhibits DPP-IV enzyme, resulting in glucose tolerance response in rats. In this study, we have explored the active phytochemicals responsible for this potential. The results have been validated in both fasting and postprandial states in the plasma of normal rats and also in fasting blood and intestinal homogenates of diabetic models. METHODS: Pueraria tuberosa water extract (PTWE) was administered to normal Charles Foster rats for 35 days and to diabetic model (65 mg/kg bw) for 10 days. After treatments, oral glucose tolerance test (OGTT) and insulin was done for 90 min, and the changes in the levels of GLP-1, GIP, and DPP-IV activities were monitored in fasting and postprandial states. In the case of the diabetic model, DPP-IV activity was measured in intestinal homogenate and basal insulin in plasma. The components of PTWE were analyzed via HPLC-MS based on their chemical formula, molecular ma...